rbpv_v21n4_a04.pdf

Trichodina colisae

: a new parasite in Brazil

Remarks

From the comparison of the 11 taxonomic characters obtained

in the measurements of 10 species of trichodinidae previously

described in the literature (Table 2), it was possible to observe

that 45.5% of the characteristics were similar to

Trichodina

nigra

Lom (1960) in the description by Basson et al. (1983) and

Trichodina pagoda

Basson and Van As (1994).

Trichodina erbilensis

Shwani et al. (2010) and

Trichodina ngoma

Van As and Basson

(1992) presented 63.6% similarity, whereas

Trichodina modesta

Lom (1970) and

Trichodina minuta

Basson et al. (1983) matched

81.8% of the measurements in this study. The trichodinids with

over 90% of observed similarities were

Trichodina kazubski

Van As

and Basson (1989) (90.9%),

Trichodina nkasa

Van As and Basson

(1992) (90.9%),

Trichodina matsu

Basson and Van As (1994),

and

Trichodina colisae

Asmat and Sultana (2005) (100% both).

Tabela 2.

Measurements (μm) of the most similar species of Trichodina with the present material. The means are followed by standard deviation,

and in parenthesis the minimum and maximum values.

Characters

Trichodina modesta

Lom (1970)

Trichodina minuta

Basson et al. (1983)

Trichodina nigra

Lom

(1960) in Basson et al. (1983)

Trichodina kazubski

Van

As and Basson (1989)

Local

Hungary

South Africa

South Africa and Israel

South Africa

Body

44.0 (34-56)

33.3 ± 3.2 (28.2-38.0)

45.9 ± 3.1 (41.7-56.7)

41.2 ± 4.5 (34.3-54.6)

Border membrane

3-3.5

3.3 ± 0.3 (2.6-4.1)

5.1 ± 0.4 (4.0-5.9)

4.3 ± 0.6 (3.2-5.9)

Adhesive disc

33.0 (28-43)

27.2 ± 3.0 (22.4-33.7)

35.1 ± 2.9 (31.7-42.6)

32.9 ± 3.7 (26.7-39.5)

Denticular ring

18 (15-20)

15.5 ± 1.7 (12.2-18.2)

20.6 ± 2.0 (18.9-23.7)

20.2 ± 2.4 (16.4-26.3)

Number of denticles

23 (22-25)

21 (19-22)

20 (19-22)

23 (22-26)

Denticle

3-3.5

4.2 ± 0.5 (3.2-5.7)

6.3 ± 0.7 (5.1-7.5)

4.3 ± 0.5 (3.6-5.7)

Blade

4-4.5

3.7 ± 0.4 (3.1-4.6)

4.6 ± 0.7 (3.3-5.9)

4.6 ± 0.7 (3.5-5.7)

Central Part

1.5

2.2 ± 0.2 (1.7-2.7)

2.5 ± 0.7 (1.6-3.0)

2.0 ± 0.4 (1.5-3.2)

Ray

3.5-4.0

3.4 ± 0.5 (2.6-4.4)

3.9 ± 0.6 (2.5-4.9)

5.2 ± 0.6 (3.6-6.4)

Pins per denticle

7-8

6 (6-7)

10 (8-11)

8 (7-10)

Adoral ciliary spiral

400-410°

380-410°

400°

Characters

Trichodina nkasa

Van As

and Basson (1992)

Trichodina ngoma

Van As

and Basson (1992)

Trichodina pagoda

Basson

and Van As (1994)

Trichodina matsu

Basson

and Van As (1994)

Local

Namibia

Taiwan

Body

34.1 ± 3.0 (29.5-39.5)

43.4 ± 6.0 (36.5-57.0)

46.5 ± 2.4 (41.0-51.0)

40.4 ± 2.7 (35.5-46.5)

Border membrane

2.8 ± 0.5 (2.5-4.0)

3.4 ± 0.4 (2.5-4.0)

4.1 ± 0.3 (3.5-5.0)

3.6 ± 0.4 (3.0-4.0)

Adhesive disc

28.5 ± 2.8 (24.5-34.5)

36.7 ± 5.7 (29.5-49.5)

38.2 ± 2.7 (32.0-43.0)

33.1 ± 3.1 (26.0-40.0)

Denticular ring

16.4 ± 1.9 (13.0-19.5)

23.1 ± 4.0 (18.0-30.5)

22.6 ± 2.1 (19.0-29.0)

19.0 ± 1.8 (15.0-22.0)

Number of denticles

20 (19-22)

23 (21-27)

22 (19-24)

21 (20-27)

Denticle span

9.5 ± 1.1 (7.5-11.5)

11.0 ± 1.5 (8.5-15.0)

13.8 ± 1.4 (12.0-16.0)

10.5 ± 0.9 (8-12)

Denticle

5.4 ± 0.7 (4.5-7.0)

5.0 ± 0.8 (3.1-6.0)

6.2 ± 0.4 (5.5-7.0)

5.5 ± 0.6 (5.0-7.0)

Blade

4.1 ± 0.5 (3.0-5.0)

4.4 ± 0.7 (3.5-6.0)

5.1 ± 0.5 (4.0-6.0)

4.2 ± 0.4 (3.5-5.0)

Central Part

1.7 ± 0.3 (1.5-2.0)

1.7 ± 0.2 (1.5-2.5)

2.2 ± 0.3 (2.0-3.0)

1.9 ± 0.2 (1.5-2.0)

Ray

3.7 ± 0.7 (2.5-5.0)

4.9 ± 1.3 (3.0-9.0)

6.4 ± 0.6 (5.0-7.5)

4.1 ± 0.4 (3.0-5.0)

Pins per denticle

7 (6-8)

10 (8-10)

8 (7-9)

7 (6-8)

Adoral ciliary spiral

400-425°

408°

410-430°

415-440°

Characters

Trichodina colisae

Asmat and Sultana (2005)

Trichodina erbilensis

Shwani et al. (2010)

Local

Bangladesh

Iraq

Body

36.9 ± 3.0 (35.8-44.9)

44.4 ± 0.4 (42-48)

Border membrane

3.7 ± 0.4 (3.1-4.6)

3.9 ± 0.2 (3-5)

Adhesive disc

32.2 ± 2.6 (28.8-36.2)

37.5 ± 0.4 (35-40)

Denticular ring

18.3 ± 1.9 (16.3-20.9)

23.6 ± 0.3 (22-27)

Number of denticles

23.1 ± 1.3 (20-24)

24.6 ± 0.3 (23-27)

Denticle span

10.5 ± 0.5 (9.9-11.2)

10.9 ± 0.2 (10-13)

Denticle

5.2 ± 0.2 (4.9-5.6)

5.4 ± 0.3 (4-7)

Blade

4.2 ± 0.6 (3.1-5.1)

4.6 ± 0.2 (4-6)

Central Part

2.0 ± 0.1 (1.5-2.1)

2.6 ± 0.1 (2-3)

Ray

4.1 ± 0.2 (3.8-4.6)

3.7 ± 0.1 (3-4)

Pins per denticle

9.1 ± 0.8 (8-10)

5.4 ± 0.3 (4-8)

Adoral ciliary spiral

diameter,

width,

length,

thickness,

length between terminations of macronucleus.

v. 21, n. 4, out.-dez. 2012

369

Jerônimo, G.T. et al.

Rev. Bras. Parasitol. Vet.

Trichodina pagoda

(Basson and Van As, 1994) and

erbilensis

(Shwani et al., 2010) are similar to the material of this study

regarding the number of denticles, pins per denticle, length of

denticle, and ray length.

ngoma

(Van As and Basson, 1992) is

similar concerning body measurement, border membrane, number

of denticles, radial pins by denticle, denticle length, central part

and ray length. On the other hand,

minuta

Basson et al. (1983)

differs from the species found in this research in denticle length

and ray length, which were smaller in this species. Similarly,

nkasa

(Van As and Basson, 1992) shows ray of smaller size,

and this species also has a prominent apophysis in the fitting of

the central part with the ray. In this research, it was possible to

observe only a discrete apophysis in some specimens.

The border membrane is broad in

kazubski

(Van As and

Basson, 1989), which can be confirmed in the description by

Tang et al. (2007) for specimens collected from cyprinids in China.

Additionally, this species differs from the others by presenting

apophysis in the anterior blade margin and prominent apophysis of

the ray (VAN AS; BASSON, 1989). In the central part,

kazubski

(Van As and Basson, 1989) shows discreet indentation below the

X axis, opposite to the ray apophysis of the posterior denticle,

which was not perceived in this research.

Two species of trichodinidae showed 100% of morphometric

similarity with the specimens analyzed in this study, namely

matsu

(Basson and Van As, 1994) and

colisae

(Asmat

and Sultana, 2005). Asmat and Sultana (2005) recognized the

similarity between these species and have set some criteria to

distinguish the two of them. Among these criteria, the following

were observed in the sample specimens, namely: 1- absence of

indentation in the inferior part of the X axis of the central part of

colisae

; 2

thick, inflated, round-tipped ray in

colisae

, while

matsu

it is thin and sharp tipped; 3-the posterior blade

margin forms a triangular curve in

matsu

, while in

colisae

it is growing and shallow. These three main characteristics of the

species based the differentiation of trichodinid species found

in the Brazilian fish of this research, coinciding with

colisae

Asmat and Sultana (2005).

Trichodina colisae

was initially reported on gills of colisa

Polyacanthus fasciatus

(synonym

Trichogaster fasciatus

), an ornamental

fish from Bangladesh. This is the second record of this species

of trichodinid in the world and the first one in Brazil. Pacu

(

mesopotamicus

) and hybrid patinga

(

mesopotamicus

brachypomus

) are new records of hosts for this parasite.

Taxonomic Summary

• Host:

Colisa

(

Polyacanthus fasciatus

) (synonym

Trichogaster

fasciatus

);

• Location:

Bangladesh;

• New

hosts:

Pacu

(

Piaractus mesopotamicus

) and hybrid

patinga

(

mesopotamicus

brachypomus

);

• New

location:

Guaíra

(20°

20’

47.1”

and

48°

11’

27.1” O)

and Jaboticabal (22° 14’ 22.7” S and 48° 17’ 33.4” O), São

Paulo, Southeast of Brazil; Dourados (22° 19’ 42.7” S and

54° 43’ 55.2” O), Mato Grosso do Sul, Central Brazil.

• Site

infestation:

Skin,

fins

and

gill.

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