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Cintia Hiromi Okino, Marcos Antônio Zanella Mores, Iara Maria Trevisol, Arlei Coldebella, Hélio José Montassier, Liana Brentano
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles
www.plosone.org
www.plosone.org
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-33.9418 -1.2982 Td
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2.5171 0 Td
(of)Tj
1.0148 0 Td
(IL6,)Tj
1.8028 0 Td
(IL1)Tj
/F11 1 Tf
()Tj
/F7 1 Tf
2.058 0 Td
(and)Tj
1.7291 0 Td
(IFN)Tj
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(\r)Tj
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(7dpi,)Tj
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-32.6832 -1.2983 Td
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1.0374 0 Td
(CD8)Tj
2.092 0 Td
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4.405 0 Td
(homolog)Tj
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(mRNA)Tj
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(2)Tj
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(].)Tj
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(chickens)Tj
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1.9956 0 Td
(IBV,)Tj
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4.0422 0 Td
(nephritis)Tj
3.7984 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
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(].)Tj
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2.6646 0 Td
(20)Tj
1.1735 0 Td
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3.3335 0 Td
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-30.4268 -1.2983 Td
(chicken)Tj
3.3278 0 Td
(line)Tj
1.7064 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
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(].)Tj
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2.2507 0 Td
(level)Tj
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1.0148 0 Td
(IL6)Tj
1.576 0 Td
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4.4617 0 Td
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(chickens)Tj
-33.8738 -1.2983 Td
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1.9956 0 Td
(a)Tj
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7.5288 0 Td
(IBV)Tj
1.8312 0 Td
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2.5001 0 Td
(and)Tj
1.7291 0 Td
(it)Tj
0.7881 0 Td
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1.542 0 Td
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1.8198 0 Td
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2.0013 0 Td
(viral)Tj
1.9899 0 Td
(load)Tj
-35.2344 -1.3039 Td
(and)Tj
1.7291 0 Td
(influx)Tj
2.5625 0 Td
(of)Tj
1.0148 0 Td
(inflammatory)Tj
5.7089 0 Td
(cells)Tj
1.9219 0 Td
(in)Tj
1.0318 0 Td
(this)Tj
1.6781 0 Td
(organ)Tj
2.5455 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
(].)Tj
-16.9964 -1.2982 Td
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3.1407 0 Td
(the)Tj
1.4684 0 Td
(development)Tj
5.4084 0 Td
(of)Tj
1.0148 0 Td
(adaptive)Tj
3.5773 0 Td
(immunity)Tj
4.2236 0 Td
(against)Tj
3.0274 0 Td
(IBV)Tj
1.8369 0 Td
(infection,)Tj
4.0138 0 Td
(the)Tj
1.4683 0 Td
(local)Tj
2.0806 0 Td
(cell-medi-)Tj
-32.4564 -1.2983 Td
(ated)Tj
1.9048 0 Td
(immune)Tj
3.6227 0 Td
(\(CMI\))Tj
2.7949 0 Td
(response)Tj
3.7361 0 Td
(was)Tj
1.7007 0 Td
(associated)Tj
4.252 0 Td
(with)Tj
2.0012 0 Td
(CD3+,)Tj
2.897 0 Td
(CD8+)Tj
2.6759 0 Td
(and)Tj
1.7291 0 Td
(CD4+)Tj
2.6759 0 Td
(cells)Tj
1.9219 0 Td
(influx)Tj
2.5625 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
-35.5122 -1.3039 Td
(trachea)Tj
3.1294 0 Td
(from)Tj
2.2053 0 Td
(3)Tj
0.6973 0 Td
(to)Tj
1.0262 0 Td
(7)Tj
0.6973 0 Td
(dpi)Tj
1.5307 0 Td
(with)Tj
1.9956 0 Td
(IBV)Tj
1.8368 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(].)Tj
2.3017 0 Td
(Furthermore,)Tj
5.6126 0 Td
(CD8)Tj
2.0976 0 Td
(and)Tj
1.7291 0 Td
(other)Tj
2.3471 0 Td
(CMI)Tj
2.1146 0 Td
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2.9481 0 Td
(genes)Tj
2.4377 0 Td
(were)Tj
-34.7071 -1.2983 Td
(markedly)Tj
3.9911 0 Td
(increased)Tj
4.0025 0 Td
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2.9253 0 Td
(secondary)Tj
4.2747 0 Td
(response)Tj
3.7303 0 Td
(related)Tj
2.948 0 Td
(to)Tj
1.0205 0 Td
(memory)Tj
3.617 0 Td
(protection)Tj
4.3823 0 Td
(induced)Tj
3.4639 0 Td
(by)Tj
1.1849 0 Td
(the)Tj
-35.5405 -1.2982 Td
(first)Tj
1.8028 0 Td
(antigen)Tj
3.2088 0 Td
(IBV)Tj
1.8311 0 Td
(exposition)Tj
4.3824 0 Td
(at)Tj
0.9524 0 Td
(1dpi)Tj
2.0069 0 Td
(\(8\).)Tj
1.6044 0 Td
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4.8983 0 Td
(the)Tj
1.4683 0 Td
(CMI)Tj
2.1146 0 Td
(genes)Tj
2.4435 0 Td
(were)Tj
2.1203 0 Td
(found)Tj
2.6192 0 Td
(most)Tj
2.2053 0 Td
(upregu-)Tj
-33.6583 -1.2983 Td
(lated)Tj
2.1486 0 Td
(later,)Tj
2.2167 0 Td
(at)Tj
0.9524 0 Td
(5dpi,)Tj
2.2337 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(primary)Tj
3.4583 0 Td
(adaptive)Tj
3.5773 0 Td
(immune)Tj
3.6226 0 Td
(response)Tj
3.736 0 Td
(of)Tj
1.0148 0 Td
(non-vaccinated)Tj
6.4063 0 Td
(chickens)Tj
3.6907 0 Td
(and)Tj
-35.5632 -1.3039 Td
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(with)Tj
1.9956 0 Td
(high)Tj
2.0069 0 Td
(virus)Tj
2.2054 0 Td
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1.9332 0 Td
(in)Tj
1.0374 0 Td
(trachea,)Tj
3.3562 0 Td
(indicating)Tj
4.269 0 Td
(this)Tj
1.6724 0 Td
(response)Tj
3.7304 0 Td
(might)Tj
2.6022 0 Td
(be)Tj
1.1508 0 Td
(more)Tj
2.3414 0 Td
(involved)Tj
3.6454 0 Td
(in)Tj
-36.1925 -1.2983 Td
(the)Tj
1.4683 0 Td
(IBV)Tj
1.8368 0 Td
(pathogenesis)Tj
5.3518 0 Td
(in)Tj
1.0375 0 Td
(this)Tj
1.6724 0 Td
(post-infection)Tj
5.828 0 Td
(period)Tj
2.829 0 Td
(of)Tj
1.0148 0 Td
(non-immune)Tj
5.5842 0 Td
(chickens)Tj
3.6907 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(].)Tj
-29.1173 -1.2983 Td
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5.2837 0 Td
(underlying)Tj
4.5978 0 Td
(immune-pathogenesis)Tj
9.1105 0 Td
(caused)Tj
2.9196 0 Td
(by)Tj
1.1792 0 Td
(IBV)Tj
1.8369 0 Td
(and)Tj
1.7291 0 Td
(those)Tj
2.3357 0 Td
(relating)Tj
3.2769 0 Td
(to)Tj
1.0261 0 Td
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-34.4917 -1.3039 Td
(clearance)Tj
3.9458 0 Td
(have)Tj
2.0636 0 Td
(begun)Tj
2.6872 0 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
(elucidated)Tj
4.32 0 Td
([)Tj
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(2)Tj
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(,)Tj
0.83 0.64 0.02 0 k
(4)Tj
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(,)Tj
0.83 0.64 0.02 0 k
(7)Tj
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(,)Tj
0.83 0.64 0.02 0 k
(8)Tj
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(,)Tj
0.83 0.64 0.02 0 k
(16)Tj
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(,)Tj
0.83 0.64 0.02 0 k
(17)Tj
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(,)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
8.4131 0 Td
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4.0762 0 Td
(there)Tj
2.2621 0 Td
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1.4513 0 Td
(no)Tj
1.2756 0 Td
(studies)Tj
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(the)Tj
1.4683 0 Td
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5.091 0 Td
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1.7348 0 Td
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6.2985 0 Td
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3.1975 0 Td
(induced)Tj
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1.0998 0 Td
(isolates)Tj
3.1181 0 Td
(differing)Tj
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1.389 0 Td
(chickens.)Tj
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1.7858 0 Td
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1.7292 0 Td
(compare)Tj
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(the)Tj
1.4683 0 Td
(pathogenesis)Tj
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(and)Tj
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(the)Tj
1.4683 0 Td
(innate)Tj
2.7383 0 Td
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-35.1947 -1.2983 Td
(adaptive)Tj
3.5773 0 Td
(immune)Tj
3.6226 0 Td
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1.1792 0 Td
(the)Tj
1.4683 0 Td
(infection)Tj
3.7928 0 Td
(with)Tj
2.0012 0 Td
(two)Tj
1.7065 0 Td
(Brazilian)Tj
3.7814 0 Td
(field)Tj
1.9729 0 Td
(isolates)Tj
3.118 0 Td
(of)Tj
1.0148 0 Td
(IBV)Tj
-34.7978 -1.2983 Td
(classified)Tj
3.8154 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(BR-I)Tj
2.1146 0 Td
(genotype.)Tj
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 343.2188 cm
BT
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1 TL
-0.0033 Tc
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(Material)Tj
3.959 0 Td
(and)Tj
1.989 0 Td
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Q
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BT
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2.0182 0 Td
(Brazilian)Tj
3.7814 0 Td
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1.9729 0 Td
(isolates)Tj
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3.6453 0 Td
(KU727196,)Tj
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(identi-)Tj
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1.8368 0 Td
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0.9015 0 Td
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3.101 0 Td
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1.7292 0 Td
(BI/BR/Embrapa/127/2006)Tj
10.6411 0 Td
(\(accession)Tj
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(KU727200,)Tj
4.7055 0 Td
(identified)Tj
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1.8312 0 Td
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0.7994 0 Td
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2.3584 0 Td
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(bronchitis)Tj
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(flocks)Tj
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(Brazil.)Tj
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1.7291 0 Td
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(five)Tj
1.6498 0 Td
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4.5694 0 Td
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3.1918 0 Td
(pathogen-free)Tj
5.7997 0 Td
(\(SPF\))Tj
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(chicken)Tj
3.3278 0 Td
(eggs/sample)Tj
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(the)Tj
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1.44 0 Td
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([)Tj
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(24)Tj
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1.7291 0 Td
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2.1997 0 Td
(titers)Tj
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1.9276 0 Td
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3.3052 0 Td
(infectious)Tj
4.1272 0 Td
(doses)Tj
2.4037 0 Td
(\(EID)Tj
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1 j
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(50)Tj
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Q
q
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(according)Tj
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1.474 0 Td
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2.211 0 Td
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(method)Tj
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(25)Tj
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(IBV)Tj
1.8311 0 Td
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1.9729 0 Td
(strains)Tj
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(automated)Tj
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(sequencing)Tj
4.7111 0 Td
(\(ABI3130XL,)Tj
5.4822 0 Td
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3.4016 0 Td
(Biosystems\))Tj
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(1059)Tj
2.1203 0 Td
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(S1)Tj
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(gene)Tj
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(ampli-)Tj
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(fied)Tj
1.7234 0 Td
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2000 Adobe Systems Incorporated. All Rights Reserved. U.S. Patent Des. 337,604.Minion is either a registered trademark or a trademark of Adobe Systems Incorporated in the United States and/or other countries.Minion Pro RegularMinion Pro% i WZ5:`v22> % I
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